對(dui)于很(hen)多(duo)科(ke)研汪(wang)來說，細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)是脆(cui)弱(ruo)又(you)重(zhong)(zhong)要(yao)的(de)(de)(de)(de)寶貝之一(yi)，承擔著重(zhong)(zhong)要(yao)的(de)(de)(de)(de)實(shi)驗數據希望(wang)。尤其是在(zai)凍存(cun)(cun)(cun)(cun)復蘇(su)的(de)(de)(de)(de)時候，經(jing)常會因為一(yi)些小細(xi)(xi)節導致問題。比(bi)如：沒(mei)有(you)控制好細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)生(sheng)長密度細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)密度對(dui)細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)生(sheng)存(cun)(cun)(cun)(cun)質(zhi)量有(you)著重(zhong)(zhong)要(yao)的(de)(de)(de)(de)影響(xiang)，畢竟它們是一(yi)群又(you)不(bu)能(neng)擠著了也不(bu)能(neng)孤(gu)獨的(de)(de)(de)(de)嬌貴寶寶。密度過(guo)高會讓細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)沒(mei)有(you)足夠的(de)(de)(de)(de)生(sheng)存(cun)(cun)(cun)(cun)空間(jian)，密度過(guo)低會讓細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)無法互相連接(jie)健康生(sheng)長。建(jian)議(yi)大(da)家在(zai)細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)接(jie)種前(qian)，一(yi)定要(yao)調整好適合(he)細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)生(sheng)長的(de)(de)(de)(de)濃度，提高細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)的(de)(de)(de)(de)存(cun)(cun)(cun)(cun)活率。溫度控制不(bu)達(da)標慢凍快融是細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍存(cun)(cun)(cun)(cun)復蘇(su)的(de)(de)(de)(de)*關鍵詞之一(yi)存(cun)(cun)(cun)(cun)細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)時需現配凍存(cun)(cun)(cun)(cun)液(ye)(如購買市面上通用(yong)的(de)(de)(de)(de)含血清(qing)(qing)細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍存(cun)(cun)(cun)(cun)液(ye)。杭州長沙無血清(qing)(qing)細(xi)(xi)胞(bao)(bao)(bao)(bao)(bao)凍存(cun)(cun)(cun)(cun)液(ye)

無(wu)(wu)(wu)血(xue)清(qing)細(xi)(xi)(xi)胞(bao)(bao)凍(dong)存(cun)(cun)(cun)液(ye)(ye)，含多(duo)種保護(hu)劑(ji)成分。一些保護(hu)劑(ji)，易于(yu)穿透(tou)細(xi)(xi)(xi)胞(bao)(bao)，避免細(xi)(xi)(xi)胞(bao)(bao)內(nei)部水分子形成冰晶損傷細(xi)(xi)(xi)胞(bao)(bao)，同時另一些保護(hu)劑(ji)不(bu)能穿透(tou)細(xi)(xi)(xi)胞(bao)(bao)，但可(ke)(ke)以在冰晶形成之前，優(you)先(xian)結(jie)合細(xi)(xi)(xi)胞(bao)(bao)外(wai)的(de)水分子，降低細(xi)(xi)(xi)胞(bao)(bao)外(wai)溶液(ye)(ye)的(de)電解質濃度(du)，減少陽離子進入細(xi)(xi)(xi)胞(bao)(bao)的(de)數量，為多(duo)種保護(hu)劑(ji)組合，在細(xi)(xi)(xi)胞(bao)(bao)內(nei)外(wai)進行雙重保護(hu)，較大(da)提高了細(xi)(xi)(xi)胞(bao)(bao)復蘇(su)存(cun)(cun)(cun)活率(lv)。無(wu)(wu)(wu)血(xue)清(qing)細(xi)(xi)(xi)胞(bao)(bao)凍(dong)存(cun)(cun)(cun)液(ye)(ye)不(bu)含牛血(xue)清(qing)，無(wu)(wu)(wu)動物源組份。2-8℃即(ji)可(ke)(ke)穩定保存(cun)(cun)(cun)，無(wu)(wu)(wu)需冷凍(dong)，即(ji)取即(ji)用(yong)。凍(dong)存(cun)(cun)(cun)細(xi)(xi)(xi)胞(bao)(bao)，無(wu)(wu)(wu)需程序降溫(wen)。凍(dong)存(cun)(cun)(cun)細(xi)(xi)(xi)胞(bao)(bao)復蘇(su)率(lv)在90%以上。重慶正規無(wu)(wu)(wu)血(xue)清(qing)細(xi)(xi)(xi)胞(bao)(bao)凍(dong)存(cun)(cun)(cun)液(ye)(ye)廠家批發(fa)價無(wu)(wu)(wu)血(xue)清(qing)細(xi)(xi)(xi)胞(bao)(bao)凍(dong)存(cun)(cun)(cun)液(ye)(ye)使用(yong)方法：加入適(shi)量的(de)無(wu)(wu)(wu)血(xue)清(qing)型細(xi)(xi)(xi)胞(bao)(bao)凍(dong)存(cun)(cun)(cun)液(ye)(ye)于(yu)離心管中(zhong)。

無(wu)血清細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)凍存(cun)(cun)(cun)液(ye)的操作規范：1、培(pei)養細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)至對數生(sheng)長(chang)晚期，顯微(wei)鏡觀察(cha)其外觀、形態(tai)、有(you)無(wu)污染(ran)等。選(xuan)擇狀態(tai)良好(hao)的細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)進(jin)行(xing)凍存(cun)(cun)(cun)操作(注：貼壁生(sheng)長(chang)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)，用(yong)(yong)胰蛋白酶(mei)消(xiao)化(hua)并用(yong)(yong)完全培(pei)養基終止消(xiao)化(hua)，進(jin)行(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)計(ji)數;懸(xuan)浮(fu)生(sheng)長(chang)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)，進(jin)行(xing)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)計(ji)數)。2、500～1000g離心5min，棄上清。3、加(jia)入適(shi)量的細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)凍存(cun)(cun)(cun)液(ye)，重懸(xuan)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)，使細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)濃度(du)達到1～10×106/ml，置(zhi)(zhi)于凍存(cun)(cun)(cun)管(guan)中(zhong)密閉。4、采(cai)取逐級降溫保存(cun)(cun)(cun)：4℃放置(zhi)(zhi)20min，-20℃放置(zhi)(zhi)30min，-70℃過(guo)夜(ye)，置(zhi)(zhi)于液(ye)氮罐(guan)中(zhong)長(chang)期存(cun)(cun)(cun)儲。注意：務必超凈工作臺內無(wu)菌操作，避免污染(ran)。雜交瘤(liu)細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)凍存(cun)(cun)(cun)時應定期復蘇(su)，以檢查細(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)的活性(xing)和分泌抗(kang)體(ti)的穩(wen)定性(xing)。

無(wu)血清細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)液通用(yong)于(yu)各(ge)(ge)種動(dong)(dong)(dong)物(wu)細(xi)(xi)胞(bao)(bao)株（tumour細(xi)(xi)胞(bao)(bao)和常規細(xi)(xi)胞(bao)(bao)），大(da)部(bu)分(fen)的(de)干(gan)細(xi)(xi)胞(bao)(bao)，凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)細(xi)(xi)胞(bao)(bao)可在-80℃長(chang)(chang)期(qi)保(bao)存(cun)(cun)(cun)(cun)(cun)(cun)。不含(han)(han)動(dong)(dong)(dong)物(wu)源(yuan)性(xing)蛋(dan)白，不含(han)(han)血清成分(fen)，可有效減(jian)少各(ge)(ge)類細(xi)(xi)菌、病毒和支原體等(deng)(deng)(deng)污染，保(bao)證(zheng)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)細(xi)(xi)胞(bao)(bao)的(de)安全(quan)。該凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)液含(han)(han)DMSO、葡萄糖等(deng)(deng)(deng)各(ge)(ge)種細(xi)(xi)胞(bao)(bao)營養(yang)成分(fen)，提(ti)高細(xi)(xi)胞(bao)(bao)存(cun)(cun)(cun)(cun)(cun)(cun)活率和活力，亦適(shi)合于(yu)無(wu)血清培養(yang)細(xi)(xi)胞(bao)(bao)和蛋(dan)白表達細(xi)(xi)胞(bao)(bao)。產品優(you)勢：1、無(wu)需程序性(xing)降溫，可直接將細(xi)(xi)胞(bao)(bao)置于(yu)-80℃凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)，凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)液無(wu)需稀(xi)釋(shi)。2、細(xi)(xi)胞(bao)(bao)可于(yu)-80℃長(chang)(chang)期(qi)保(bao)存(cun)(cun)(cun)(cun)(cun)(cun)。3、凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)液不含(han)(han)血清等(deng)(deng)(deng)，較(jiao)大(da)降低細(xi)(xi)胞(bao)(bao)污染的(de)可能性(xing)。保(bao)存(cun)(cun)(cun)(cun)(cun)(cun)方(fang)法(fa)：冰袋運輸，4℃保(bao)存(cun)(cun)(cun)(cun)(cun)(cun)，保(bao)質期(qi)一年。無(wu)血清細(xi)(xi)胞(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)(cun)(cun)液可用(yong)于(yu)其他類型哺乳動(dong)(dong)(dong)物(wu)細(xi)(xi)胞(bao)(bao)的(de)儲(chu)存(cun)(cun)(cun)(cun)(cun)(cun)。

無血清(qing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)的使(shi)用(yong)方(fang)法及注意事項(xiang)：無血清(qing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)：含多種(zhong)保(bao)(bao)護(hu)劑(ji)成(cheng)分(fen),一些(xie)保(bao)(bao)護(hu)劑(ji),易(yi)于(yu)(yu)穿(chuan)透細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao),避免細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)內部水分(fen)子(zi)形成(cheng)冰晶損傷細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao),同時另(ling)一些(xie)保(bao)(bao)護(hu)劑(ji)不能(neng)穿(chuan)透細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao),但(dan)可(ke)以在冰晶形成(cheng)之前,優先結合(he)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)外(wai)的水分(fen)子(zi),降低細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)外(wai)溶液(ye)(ye)的電解質濃度,減少陽離(li)子(zi)進入細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的數(shu)量,我公司(si)無血清(qing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye),為多種(zhong)保(bao)(bao)護(hu)劑(ji)組合(he),在細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)內外(wai)進行(xing)雙重(zhong)保(bao)(bao)護(hu),較大提(ti)高了細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)復蘇存(cun)(cun)(cun)(cun)活(huo)率。【產品用(yong)途】1.用(yong)于(yu)(yu)免疫(yi)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)及干細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)的存(cun)(cun)(cun)(cun)儲。2.細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)保(bao)(bao)藏中心，用(yong)于(yu)(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)株的長期保(bao)(bao)存(cun)(cun)(cun)(cun)。3.科研高校，細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)株凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)。4.醫院樣本(ben)庫細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)樣本(ben)保(bao)(bao)存(cun)(cun)(cun)(cun)。【使(shi)用(yong)方(fang)法】1、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)前準備（1）要(yao)(yao)求凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)的細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)在對數(shu)生長期，且活(huo)率大于(yu)(yu)90%。（2）計算細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)密度，一般(ban)要(yao)(yao)求密度在1-3x10cells/mL，不同細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)系請參照附表。2、細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)過(guo)程（1）將要(yao)(yao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)的細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)懸液(ye)(ye)，進行(xing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)計數(shu)，計數(shu)后(hou)離(li)心，8003min即可(ke)。度保(bao)(bao)存(cun)(cun)(cun)(cun)的無血清(qing)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)緩慢加入離(li)心后(hou)的細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)沉淀中，根(gen)據密度調整(zheng)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)用(yong)量。（3）反復吹吸混勻后(hou)，分(fen)裝于(yu)(yu)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)管(guan)中，每(mei)管(guan)500ul-1000ul，（建議500ul/管(guan)）。無血清(qing)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)使(shi)用(yong)方(fang)法：將凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)管(guan)直接轉移至-80C水箱中冷(leng)凍(dong)(dong)(dong)保(bao)(bao)存(cun)(cun)(cun)(cun)。廣州正(zheng)規無血清(qing)細(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)(xi)胞(bao)(bao)(bao)(bao)凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)(ye)廠家直銷

可以(yi)防止或減少冷凍冰晶對細胞(bao)的損傷作用(yong)。杭(hang)州長沙無血清細胞(bao)凍存液

無(wu)(wu)血(xue)清快速細(xi)胞凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)，通用(yong)于各(ge)種動(dong)物細(xi)胞株。特別配方具有有效(xiao)提高(gao)(gao)細(xi)胞凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)活(huo)率(lv)和(he)復蘇(su)活(huo)力。不含動(dong)物來源性(xing)蛋白，能(neng)減少各(ge)類病毒(du)、霉菌和(he)支原(yuan)體(ti)等的污染(ran)，確(que)保凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)細(xi)胞安全。既適(shi)(shi)用(yong)于一(yi)般(ban)培養細(xi)胞的凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)，也(ye)適(shi)(shi)用(yong)于無(wu)(wu)血(xue)清培養細(xi)胞和(he)蛋白表達(da)細(xi)胞的凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)。產品(pin)特色：高(gao)(gao)安全性(xing)，完(wan)全使用(yong)醫藥(yao)品(pin)等級(ji)原(yuan)料進(jin)行(xing)生(sheng)產，不含動(dong)物成分，病毒(du)、霉菌和(he)支原(yuan)體(ti)等污染(ran)可(ke)(ke)能(neng)性(xing)低，各(ge)批(pi)產品(pin)之間(jian)有更高(gao)(gao)的產品(pin)質量一(yi)致性(xing)。細(xi)胞存(cun)(cun)(cun)(cun)活(huo)率(lv)高(gao)(gao)，無(wu)(wu)批(pi)次差異。完(wan)全凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)配方，可(ke)(ke)直接(jie)使用(yong)，方便簡捷，可(ke)(ke)直接(jie)存(cun)(cun)(cun)(cun)放(fang)于-70℃冰箱凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)，無(wu)(wu)需(xu)經過(guo)費時(shi)的程序降(jiang)溫(wen)過(guo)程（省(sheng)(sheng)(sheng)時(shi)、省(sheng)(sheng)(sheng)力、省(sheng)(sheng)(sheng)錢）。杭(hang)州(zhou)長沙(sha)無(wu)(wu)血(xue)清細(xi)胞凍(dong)(dong)(dong)存(cun)(cun)(cun)(cun)液(ye)